Doctor of Philosophy (Chemical Engineering)
Virginia Polytechnic Institute and State University
Master of Science (Chemical Engineering)
University of Iowa
Bachelor of Technology (Chemical Engineering)
Nagpur University (India)
Employment
2010-Present
Professor of Chemical & Biomolecular Engineering,
University of Nebraska-Lincoln
2005-2010
Associate Professor of Chemical & Biomolecular Engineering,
University of Nebraska-Lincoln
2001-2005
Assistant Professor of Chemical Engineering,
University of Nebraska-Lincoln
1996-2001
Assistant Professor of Biosystems Engineering,
University of Minnesota, St. Paul
Publications
Guha Thakurta, S. and Subramanian, A. Platelet response on in-situ albumin binding surfaces under conditions of low-shear flow. (Journal of Biomedical Materials 2011).
Guha Thakurta, S. and Subramanian, A. Effect of silanization of the distribution of adsorbed biomolecules (Journal of Colloid and Interface Science 2011)
Guha Thakurta, S. and Subramanian, A. Evaluation of in situ albumin binding surfaces: a study of protein adsorption and platelet adhesion. Journal of Materials Science: Materials in Medicine, (2011) 22/1, 137.
Hasanova, G. I., Noriega, S. E., Mamedov, T. G., Guha Thakurta, S., Turner, J. A., Subramanian, A. Stimulation of the Gene and Protein Expression of Chondrocytes Seeded in Chitosan Scaffolds by Ultrasound. Journal of tissue engineering and regenerative medicine, (2011) DOI: 10.1002/term.384.
Guha Thakurta, S., Viljoen, H. J., Subramanian, A. Evaluation of the real-time protein adsorption kinetics on albumin-binding surfaces by dynamic in situ spectroscopic ellipsometry. Thin Solid Films, (2011) DOI: 10.1016/j.tsf.2011.09.084.
Hasanova, G., Mamedov, T. and Subramanian, A. A study in the gene expression upon the
indirect mechanical stimulation via ultrasound of chondrocytes seeded in scaffolds.
Journal of Tissue Engineering and Regenerative Medicine. (2011). Available online.
DOI: 10.1002/term.384.
2010
Guha Thakurta, S., Miller, R. and Subramanian, A. Investigation of Platelet Responses and Clotting Characteristics of in situ Albumin Binding Surfaces. Journal of Biomedical Application,(2010) DOI: 10.1177/0885328210377535.
TerMaat, J. A., Mamedov T.G., Pienaar, E., Whitney, S.E. and Subramanian, A. Automated two-column purification of Iminobiotin and BrdU labeled PCR products for rapid cloning: application to genes synthesized by Polymerase Chain Assembly. J.Chromatographic Science., Vol. 48, pp 1-5, January 2010.
Kador, K. E. and Subramanian, A. Controlled and Sequential Immobilization of Proteins onto Biomedical Grade Polyurethane. (In Press: Journal of Biomaterial Science-Polymer Edition 2010. DOI:10.1163/092050610X529191).
Kador, K. E. and Subramanian, A. Sequential Immobilization of recombinant EPCR and thrombomodulin onto Biomedical Grade Polyurethane. (In press: Acta Biomaterialia 2010. DOI:10.016/j.actbio.2011.03.015).
Noriega, S, Hasanova, G, Larsen, G.F. and Subramanian, A. Effect of fiber diameter on spreading, proliferation and differentiation of chondrocytes on electrospun chitosan matrices (In Press: Cells, Tissues and Organs 2010, DOI: 10.1159/000325144).
Skotak, M., Noriega, S., Larsen, G., and Subramanian, A. Electrospun cross-linked gelatin
fibers with controlled diameter: The effect of matrix stiffness on proliferative and
biosynthetic activity of chondrocytes cultured in vitro. [In Press: Journal of Biomedical
Materials Research, 2010, published online: 7 SEP 2010, DOI: 10.1002/jbm.a.3285].
Noriega, S and Subramanian, A. Chitosan as cell culture scaffold material: consequences of scaffold pretreatment on chondrocyte proliferation, attachment and cytoskeletal organization. (Journal of Biomedical Materials 2009).
Noriega, S, Hasanova, G, Larsen, G.F. and Subramanian, A. Effect of fiber diameter on spreading, proliferation and differentiation of chondrocytes on electrospun chitosan matrices (Journal of Biomedical Materials 2009).
Noriega, S, Schneider, M, Larsen, G. and Subramanian, A. Effect of time of culture on protein expression of chondrocytes seeded on chitosan and gelatin based scaffolds. (Journal of Biomedical Materials 2009).
Noriega, S. Hasanova, G. and Subramanian, A. Effect of US stimulation on the cytoskeletal organization and distribution of chondrocytes seeded on 3D scaffolds.(Matrix Biology 2009).
Noriega, S and Subramanian, A. Characterization of the bioeffects of ultrasound stimulation on cell seeded constructs. (BMC Biotechnology 2009).
Kador, K. E. and Subramanian, A. Sequential Immobilization of recombinant EPCR and thrombomodulin onto Biomedical Grade Polyurethane. (Thrombosis Research 2009).
TerMaat, J.R., Pienaar, E., Whitney, S. E., Mamedov, T. G. and Subramanian. A. Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high speed thermocycler. Journal of Microbiological Methods, 2009. (doi:10.1016/j.mimet.2009.09.015).
Goyal, D. K. and Subramanian, A. In-situ Protein Adsorption Study on Biofunctionalized Surfaces Using Spectroscopic Ellipsometry. Thin Films. 2009. (doi:10.1016/j.tsf.2009.10.110)
Pattanayak, A. and Subramanian, A. Impact of porogens on the pore characteristics of zirconia particles made by Polymer Induced Colloid Aggregation (PICA). Int. J. Appl. Ceram. Technol., 18 (2009).
(DOI:10.1111/j.1744-7402.2009.02410.x).
Pattanayak, A. and Subramanian, A. Production of Meso- and Giga-Porous Zirconia Particles – An Improved Multi-Step Particle Aggregation Process. Powder Technology 192 (2009) 359–366).
2008
Mammedov, T.G., Pienaar, E., Whitney. S. E., TerMaat, J. R., Carvill. G., Goliath. R., ,Subramanian,. A. and Viljoena. H. J. A fundamental study of the PCR amplification of GC-rich DNA templates. Comput Biol
Chem (2008), doi:10.1016/j.compbiolchem.2008.07.021.
Skotak, M., Leonov, A. P., Larsen, G., Noriega, S. and Subramanian, A. Biocompatible and Biodegradable Ultrafine Fibrillar Scaffold Materials for Tissue Engineering by Facile Grafting of L-Lactide onto Chitosan. Biomacromolecules (2008), 9(7), 1902-1908.
Goyal. D.K., Pribil, G.K., J. A. Woollam, and A. Subramanian. Detection of ultrathin biological films using vacuum ultraviolet spectroscopic ellipsometry. Materials Science and Engineering B 149 (2008) 26–33
Brewster, L. P., Washington, C., Brey, E.M., Gassman, A., Subramanian, A., Velander, W.H. and Griesler, H.P. Construction and characterization of a thrombin-resistant designer FGF-based collagen binding domain angiogen. Biomaterials. 29, (2008), 327-336.
2007
Guha Thakurta, S. and Subramanian, A. Effect of Hydrofluoric Acid in Oxidizing Acid Mixtures on the Hydroxylation of Silicon Surface. Journal of The Electrochemical Society, (2007) 154/11, P136.
Mammedov, T., Kotera, C., Padhye, N., Viljoen, H. V. and Subramanian, A. “Rational Design of “de-novo” gene synthesis: synthesis of native and deglycosylated endothelial protein C receptor”. J. Biotechnology, 131 (2007) 378-387.
Parampalli, A., Mowry, M.A., Meagher, M. M. and Subramanian, A. Development of serum-free media in CHO-DG44 cells using central composite design. J.Cytotechnology. 2007. 54, 57-68.
Noriega, S., Mamedov, T., Turner, J. A., and Subramanian, A. “Effect of Intermittent Applications of Continuous Ultrasound on the Viability, Proliferation, Morphology and Matrix Production of Chondrocytes in 3-D Matrices”. Tissue Engineering. Vol 13(3). 2007.
Nosal, W. H.; Thompson, D. W.; Tiwald, T. E.; Sarkar, S.; Subramanian, A.; Woollam, J. A. Vacuum ultraviolet optical analysis of spin-cast chitosan films modified by succinic anhydride and glycidyl phenyl ether. Surface and Interface Analysis (2007), 39(9), 747-751.
2006
Subramanian, Anu; Martinez, Blanca; Holm, Jill; Carr, Peter; McNeff, Clayton. A Comparative Study of Monoclonal Antibodies (Mabs) Purified from Cell Culture Supernatant on EDTPA-Modified Zirconia Beads and Protein A-Hyper D Support. Journal of Liquid Chromatography & Related Technologies (2006), 29(4), 471-484.
Subramanian, Anuradha; Rau, Anand V.; Kaligotla, Hemanth. Surface modification of chitosan for selective surface-protein interaction. Carbohydrate Polymers (2006), 66(3), 321-332.
2005
Nosal, W. H.; Thompson, D. W.; Sarkar, S.; Subramanian, A.; Woollam, J. A. Quantitative oscillator analysis of IR-optical spectra on spin-cast chitosan films. Spectroscopy (Amsterdam, Netherlands) (2005), 19(5-6), 267-274.
Nosal, W. H.; Thompson, D. W.; Yan, L.; Sarkar, S.; Subramanian, A.; Woollam, J. A. UV-vis-infrared optical and AFM study of spin-cast chitosan films. Colloids and Surfaces, B: Biointerfaces(2005), 43(3-4), 131-137.
Nosal, W. H.; Thompson, D. W.; Yan, L.; Sarkar, S.; Subramanian, A.; Woollam, J. A. Infrared optical properties and AFM of spin-cast chitosan films chemically modified with 1,2 Epoxy-3-phenoxy-propane. Colloids and Surfaces, B: Biointerfaces (2005).
Nosal W H; Thompson D W; Yan L; Sarkar S; Subramanian A; Woollam J A Infrared optical properties and AFM of spin-cast chitosan films chemically modified with 1,2 Epoxy-3-phenoxy-propane. Colloids and surfaces. B, Biointerfaces (2005), 46(1), 26-31.
Nosal W H; Thompson D W; Yan L; Sarkar S; Subramanian A; Woollam J A UV-vis-infrared optical and AFM study of spin-cast chitosan films. Colloids and surfaces. B, Biointerfaces (2005), 43(3-4), 131-7.
Subramanian, Anuradha; Lin, Hsin-Yi. Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation. Journal of Biomedical Materials Research, Part A (2005), 75A(3), 742-753.
Subramanian, A. Chromatographic purification of MAbs with non-affinity supports: purifying MAbs with novel supports as well as protein A. BioPharm International (2005), 18(6), 64-70.
Sarkar, S. and Subramanian, A. Identification of the mass transfer mechanisms involved in the transport og hIgG in ETPA-modified zirconia. Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences (2005), 821(1), 124-131.
Sarkar, S. and Subramanian, A. Modeling of immunoglobulin uptake by N,N,N',N'-ethylenediaminetetramethylenephosphonic acid-modified zirconia particles under static and dynamic conditions. Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences (2005), 821(1), 81-87.
Subramanian, A., Lin, H., Vu, D and Larsen, G. F. “Synthesis and Evaluation of Scaffolds for Potential Use in Cartilage Tissue Engineering”, Journal of Biomaterial Science-Polymer Edition, :1-13 (2005).
Subramanian, A., and Hommerding, J. “The Use of Confocal Laser Scanning Microscopy to Study the Transport of Biomacromolecules in a Macroporous Support”. Journal of Chromatography-B, 818/1:80-91 (2005).
2004
Subramanian, A., Lin, H., Vu, D and Larsen, G. F. “Synthesis and Evaluation of Chitosan based scaffolds”, Biomedical Sciences Instrumentation, 40:111-117 (2004).
Subramanian, A., Sarkar, S., Woollam, J. A. and Nosal, W. “Synthesis and Characterization of Albumin Binding Surfaces for Implantable Devices”. Biomedical Sciences Instrumentation, 40:1-7 (2004)
Kunjachan, V., Subramanian, A., Hanna, M and Guan, J.J. “Comparison of different fabrication techniques used for processing 3-D, porous, biodegradable scaffolds from modified starch for use in tissue engineering”. Biomedical Sciences Instrumentation, 40:129-136 (2004).
Subramanian, A., Mascoli, C., Roy, S. K., and Hommerding, J., “The Use of Modified Chitosan Macrospheres in the Selective Removal of Immunoglobulins,” Journal of Liquid Chromatography, 24(17) (2004) 2649-2670.
Subramanian, A., and Hommerding, J., “Interaction of Immunoglobulins with Modified Chitosan,” Journal of Liquid Chromatography, 24(17) (2004) 2671-2688.
Mowry, M. C., Meagher, M. M., Smith, L., Marks, J. and Subramanian, A. “Production and purification of a chimeric monoclonal antibody against botulinum neurotoxin serotype A” Protein Expression and Purification. Vol 37/2 pp 399-408. (2004).
2003
Subramanian, A., and Sarkar, S., “Interaction of IgG with EDTPA-modified zirconia particles,” Journal of Chromatography A, 989, p. 131-138 (2003).
Subramanian, A., Carr, P. W., McNeff, C. V., and Sarkar, S., “Characterization and Optimization of a Chromatographic Process based on EDTPA-modified zirconia particles,” Journal of Chromatography. 790, 143-152 (2003).
Subramanian, A. and Sarkar, S., “The Use of EDTPA-Modified Zirconia in the Separation of Immunoproteins,” Journal of Chromatography A, ½, pp. 179-187 (2002).
2000
Subramanian, A., McNeff, C. M., and Carr, P. W., “The Use of Spray Dried Zirconia Microspheres in the Separation of Immunoglobulins from Cell Culture Supernatant,” Journal of Chromatography A, 890, p. 15-23 (2000).
Subramanian, A., and Martinez, B., “The Use of DVS activated cellulose beads in large-scale bioprocess chromatography: purification of human antibodies,” International Journal of Bio-Chromatography, 5(1), pp. 31-47 (2000).
Subramanian, A., and Velander, W. H., “Ranking the Factors Impacting Immunosorbent Performance,” International Journal of Bio-Chromatography, 5(1), pp. 67-82 (2000).
1999
Van Cott, K.E., Butler, S.P., Russell C.G., Subramanian, A., Lubon, H., Gwazdauskas, F.C., Knight, J.E., Drohan W.N., and Velander, W.H., “Transgenic Pigs as Bioreactors: A Comparison of -Carboxylation of Glutamic Acid in Recombinant Human Protein C and Factor IX by the Mammary Gland,” Genetic Analysis 15(3-5), pp. 155-60 (Nov. 1999).
Rautenschlein, S., Subramanian, A., and Sharma, J. S., “Bioactivities of a tumour necrosis –like factor released by chicken macrophages,” Development and Comparative Immunology, 23, pp. 629-640 (1999).
Subramanian, A., and Morcol, T., “A Red-Dot-Blot Protein Assay Technique in the Low Nanogram Range; Analytical Biochemistry, 270, pp. 75-82 (1999).
Clausen, A. M.; Subramanian, A., and Carr, P. W., “Characterization and Use of a Modified Zirconia Support for Monoclonal Antibody Purification,” Journal of Chromatography A, 831, pp. 63-72 (1999).
1997
Morcol, T., Subramanian, A., and Velander, W. H., “Dot-blot analysis of the degree of modification of proteins and antibodies at their amino groups,” Journal of Immunological Methods, 203, pp. 45-53 (1997).
Butler, S.P., Van Cott, K.E., Subramanian, A., Gwazduaskas, F.C., Velander, W.H., “Current Progress in the Production of Recombinant Human Fibrinogen in the Milk of Transgenic Animal,” Thrombosis and Haemostasis, 78, pp. 537-542 (1997).
Subramanian, A., Butler, S.P.; Gwazdauskas, F.C., Lubon, H., and Velander, W.H., “Expression of Human Fibrinogen in Transgenic Mice,” Proceedings of the Sixteenth Southern Biomedical Engineering Conference, pp. 53-55, 1997.
1992-1996
Subramanian, A., and Velander, W.H, “The Effect of Antibody Orientation on Immunosorbent Performance,” Journal of Molecular Recognition, 9, pp. 528-535 (1996).
Subramanian, A., Paleyanda, R.P.; Morcol, T., Gwazdauskas, F.C.; Lubon, H., and Velander, W.H., “Rate-limitation in Post-Translational Processing by the Mammary Gland of Transgenic Animals,” Annal. New York Academy of Sciences, 782, pp. 87-96 (1996).
Page, R.L., Butler, S.P., Subramanian, A., Gwazdauskas, F.C., Johnson, J.L., and Velander, W.H., “Transgenesis in Mice by Cytoplasmic Injection of Polylysine/DNA Mixtures,” Transgenic Research, 4, pp. 353-360 (1995).
Subramanian, A., Van Cott, K.E., Milbrath. D.S., and Velander, W.H., “The Role of Local Antibody Density on Immunosorbent Performance,” Journal of Chromatography, 672, pp. 11-24 (1994).
Velander, W.H., Johnson, J.L., Page, R.L., Russell, C.G., Subramanian, A., Wilkins, T.D., Gwazdauskas, F.C., Pittius, C., and Drohan, W.N., “High Level Expression in the Milk of Transgenic Swine Using the cDNA Encoding Human Protein C.,” Proceedings of the National Academy of Sciences, 89, pp. 12003-12007 (1992).
Velander, W.H., Madurawe, R.D., Subramanian, A., and Orthner, C., “Polyoxazoline Adducts which Retain Antibody Activity,” Biotech. and Bioeng, 39, pp. 1024-1030 (1992).
Velander, W.H., Subramanian, A., Madurawe, R.D., and Orthner, C., “The Use of Fab Masking Antigens to Enhance The Activity of Immobilized Antibodies,” Biotech. and Bioeng, 39, pp. 1013-1023 (1992).
Velander, W.H., Subramanian, A., Degener, A.D., Morcol, T., Johnson, J.L., Gwazdauskas, F.C., Lubon, H., and Drohan, W.N., “Expression of Human Protein C in Transgenic Swine,” ACS Conference Proceedings, Chapter 9 (1992).
Book Chapters
"General Considerations in Affinity Chromatography", Anu Subramanian, Editor D.S.Hage. (in press: 2004, Marcel and Dekker Inc.,).
"Purification of recombinant proteins by affinity chromatography", Anu Subramanian, Editor D.S.Hage. (in press: 2004, Marcel and Dekker Inc.,).
"Immunoaffinity Chromatography", in Protocols in Molecular Biology, published by Humana Press Inc., 2001.
"Immunoaffinity Chromatography", in Affinity Chromatography: Methods and Protocols, published by Humana Press Inc., 2000.
"Moving to Commercialization: Transgenic Pigs and Food Production", Schook, L.B., Beattie, C.W., Subramanian, A., and Chiapetta, J. International Workshop on Transgenic Animals and Food Production, May 22-24, 1997 (Stockholm, Sweden).
Patents
Immunosorbents with Improved Binding Efficiency and Method of Manufacture. 1999. U.S. Serial No. 07/614,663 (W.H. Velander and A. Subramanian).
Method of Coupling Ligands onto Supports and Products Produced Therefrom. U.S. Serial No. 9480/81-1551, 49451USA2A (W.H. Velander, C. Orthner and A. Subramanian).
Professional Service
NSF-Workshop on Bio-Nano manufacturing, Arlington, VA, April 16-18 2008
Chair, Topical session: “Women in Management”. AICHE-2004, Austin, TX.
Organizer and Chair, 17th Annual Nebraska Biomedical Engineering Workshop, May 1st, 2004, Lincoln, NE.
NIH-RC1 Grant Applications. Mail-in-Reviewer. June 2009.
American Institute of Biological Sciences “Armed Forces Institute of Regenerative Medicine-2008”, Dec 10-11 2008.
Panel Chair.NSF Separations and Purification panel, May 8 2007
NIH “NANOSCIENCE AND NANOTECHNOLOGY IN BIOLOGY AND MEDICINE, July 8-9, 2007
NIH “NANOSCIENCE AND NANOTECHNOLOGY IN BIOLOGY AND MEDICINE, February 22-23 2007
NIH “NANOSCIENCE AND NANOTECHNOLOGY IN BIOLOGY AND MEDICINE, November 14-15, 2005
National Institutes of Health, Muscoskeletal Tissue Engineering Panel, November 2005
National Science Foundation-Bioengineering and Environmental Sciences (Panelist), March and June 2004.
NER and Sensor Network Panel.
National Science Foundation-Chemical and Transport and Separations (Panelist), May 2003.
National Science Foundation-Adhoc reviewer.
Lectures
“Understanding the impact of scaffold topography and, indirect mechanical stimulus on the cellular activity of chondrocyte maintained in scaffolds” June 11, 2008. Wellman Institute of Photomedicine, Harvard Medical School, Boston, MA.
“Downstream Processing: Recent Advances in Bioseparations”, Biophex-2004, September 28th, 2004, San Francisco, CA (Invited lecture).
“Future applications to the adsorption of large biomolecules”, SBCN 2004, International Symposium on BioChromatography, May 5 to 7th 2004, Bordeaux, France (Invited lecture).
