Angela K. Pannier

Contact Information:

East Campus (Lincoln)
368 Morrison Life Science Center
402-472-0896
apannier2@unl.edu
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Personal Links:

Professional Memberships:


Swarts Family Chair of Biological Systems Engineering
Professor of Biomedical Engineering

Academic Degrees

  • Ph.D., Biological Sciences, Northwestern University
  • M.S., Biological Systems Engineering, University of Nebraska-Lincoln
  • B.S., Biological Systems Engineering, University of Nebraska-Lincoln

Certifications

  • Ph.D.

Appointment

  • 80% Reseach
  • 20% Teaching

Curriculum Vitae (CV):

Angela Pannier CV

Areas of Research and Professional Interest

  • Nonviral Gene Delivery
  • Tissue Engineering
  • Developmental Biology
  • Stem Cell Engineering
  • Extracellular Vesicles
  • DNA vaccination
Courses Currently or Previously Taught

In the News

Inventions/Patents

  • Segura T, Shea LD, Pannier AK, Bengali Z, Jang JH, Chung P, and Anderson B. Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No. 7,029,697. April 18, 2006.

  • Pannier AK, Schubert M, Hofmann T, Kasputis TJ, Herzinger CM, Woollam JA. Method to obtain micrographs of transparent or semi-transparent specimens using anisotropy contrast. U.S. Patent No. 10,026,167. July 17, 2018.

About Angela K. Pannier

Angela K. Pannier is the Swarts Family Chair in Biological Systems Engineering, and a Professor of Biomedical Engineering, with a Courtesy Appointment in the Mary and Dick Holland Regenerative Medicine Program at the University of Nebraska Medical Center. Pannier’s research is focused in engineering systems and biomaterials for cell and gene therapies. Pannier has received funding from the American Heart Association (Scientist Development Grant), National Science Foundation (CAREER Award and MRIs), NIH (Director’s New Innovator Award), the U.S. Department of Agriculture, and industry. Pannier is active in many societies (BMES, AIChE, ASGCT), serves as a reviewer for over 50 different journals and funding agencies (including NIH and NSF), serves as an Associate Editor for Science Advances, and serves on the editorial board for two journals: Experimental Biology and Medicine and Regenerative Medicine Frontiers. In 2019 Pannier was awarded the Presidential Early Career Award for Scientist and Engineers from the U.S. White House Office of Science and Technology Policy; Pannier is the first Nebraskan to receive this honor. Most recently, Pannier was awarded the 2020 Gamma Sigma Delta Outstanding Research Award and the 2020 College of Engineering Outstanding Research and Creative Activity award. Pannier has also been awarded the college-wide Outstanding Graduate Mentor Award and the university-wide Outstanding Undergraduate Research Mentor Award (she has mentored over 50 undergraduates in her lab), as well as various teaching and service awards.  In 2020 Pannier was named a BMES Fellow and in 2022 inducted into the College of Fellows in AIMBE. Pannier has also served on and led various departmental, college and university committees, and co-chaired the university-wide N2025 Strategic Planning team in 2019.  She completed the Big Ten Academic Alliance Academic Leadership Program Fellow for the 2019-2020 academic year.  In 2020-2021, Pannier created and delivered outreach materials for dissemination of COVID-19 vaccine information.

Honors and Awards

  • Fellow, Biomedical Engineering Society (BMES) (2020)
  • College of Fellows, American Institute for Medical and Biological Engineering (AIMBE) (2022)
  • UNL College of Engineering Faculty Service Award (2022)
  • Big Ten Academic Alliance Academic Leadership Program Fellow (2019-2020)
  • UNL College of Engineering Faculty Research and Creative Activity Award (2020)
  • UNL Gamma Sigma Delta Outstanding Researcher Award (2020)
  • UNL College of Engineering Holling Family Master Teacher Award (2019)
  • Presidential Early Career Award for Scientist and Engineers (PECASE, awarded in 2019 for 2017)
  • UNL Parents Association Teaching Award (2018)
  • National Institutes of Health Director’s New Innovator Award (2017)
  • UNL Outstanding Undergraduate Research Mentor Award (2017)
  • UNL College of Engineering Tau Beta Pi Distinguished Teaching Award (2017)
  • UNL Darrell W. Nelson Excellence in Graduate Student Advising Award (2016)

Selected Publications

Refereed Journal Articles

  1. Walsh SC, Miles JR, Keel BN, Rempel LA, Wright-Johnson EC, Lindholm-Perry AK, Oliver WT, and Pannier AK. 2022. Global analysis of differential gene expression within the porcine conceptus transcriptome as it transitions through spherical, ovoid, and tubular morphologies during the initiation of elongation (in press at Mol. Reprod. Develop).
  2. Kozisek T, Hamann A, Samuelson L, Fudolig M, Pannier AK. 2021. Comparison of Promoter, DNA Vector, and Cationic Carrier for Efficient Transfection of Human Mesenchymal Stem Cells from Multiple Donors and Tissue Sources, Molecular Therapy-Nucleic Acids. 26:81-93.
  3. Lampe AT, Farris EJ, Brown DM, Pannier AK. 2021. High- and low-molecular-weight chitosan act as adjuvants during single-dose influenza A virus protein vaccination through distinct mechanisms. Biotechnology and Bioengineering. 118 (3): 1224-1243.
  4. Hamann A, Kozisek T, Broad K, Pannier AK. 2020. Glucocorticoid Priming of Nonviral Gene Delivery to Human Mesenchymal Stem Cells Increases Transfection by Reducing Induced Stresses. Molecular Therapy – Methods and Clinical Development. 18:713-722.Lampe AT, Lal Puniya B, Pannier AK, Helikar T, Brown DM  2020. Combined TLR4 and TLR9 agonists induce distinct phenotypic changes in innate immunity in vitro and in vivo. Cellular Immunology. 355:104149.
  5. Kozisek T, Hamann A, Nguyen A, Miller M, Plautz S, Pannier AK. 2020. High-Throughput Screening of Clinically Approved Drugs that Primer Nonviral Gene Delivery to Human Mesenchymal Stem Cells (Epub ahead of print).
  6. Hamann A, Thomas AK, Kozisek T, Farris E, Lück S, Zhang Y, Pannier AK. 2020. Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection. Experimental Biology and Medicine 245(7): 606-619.
  7. Walsh SC, Miles JR, Yao L, Broeckling CD, Rempel LA, Wright-Johnson EC, Pannier AK. 2020. Metabolic compounds within the porcine uterine environment are unique to type of conceptus present during the early stages of blastocyst elongation. Molecular Reproduction and Development 87(1):174-190.
  8. Hamann A, Nguyen A, Pannier AK. 2019. Nucleic acid delivery to mesenchymal stem cells: a review of nonviral delivery methods and applications. Journal of Biological Engineering. 13:7. 
  9. Mantz A, Rosenthal A, Farris E, Kozisek T, Bittrich E, Nazari S, Schubert E, Schubert M, Stamm M, Uhlmann P, Pannier AK. 2019. Free polyethylenimine enhances substrate-mediated gene delivery on titanium substrates modified with RGD-functionalized poly(acrylic acid) brushes. Frontiers in Chemistry: Poly. Chemistry. 7:51.
  10. Mantz A and Pannier AK. 2019. Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery. Experimental Biology and Medicine. 244(2):100-113.
  11. Hamann A, Broad K, Nguyen A, Pannier AK. 2019. Mechanisms of unprimed and dexamethasone-primed nonviral gene delivery to human mesenchymal stem cells. Biotechnology and Bioengineering. 116(2):427-443.
  12. Farris E, Heck K, Lampe AT, Brown DM, Ramer-Tait A, and Pannier AK. 2018. Oral non-viralgene delivery for applications in DNA vaccination and gene therapy. Current Opinion in Biomedical Engineering. 7:51-57
  13. Erickson AG, Laughlin TD, Romereim SM, Sargus-Patino CN, Pannier AK, Dudley AT. 2018. A tunable, three-dimensional in vitro culture model of growth plate cartilage using alginate hydrogel scaffolds.  Tissue Engineering, Part A. 24(1-2): 94-105.
  14. Rosenthal A, Mantz A, Bittrich E, Schubert E, Schubert M, Stamm M, Pannier AK, Uhlmann P. 2018. Biofunctionalization of Titanium Substrates Using Nanoscale Polymer Brushes with Cell Adhesion Peptides. J Physical Chemistry: B. 122 (25): 6543-6550.