NIU Publications

43. Mueller, J.; Willett, H.; Feist A.; Niu, W.* “Engineering Pseudomonas putida for syringyl aromatics utilization”, Biotechnol. Bioeng. 2022.

42. Lim, H. G.; Rychel, K.; Sastry, A. V.; Mueller, J.; Niu, W.; Feist, A. M.; Palsson, B. O.*Machine-learning from Pseudomonas putida transcriptomes reveals its transcriptional regulatory networkMetab. Eng. 2022, 72, 297-310.

41. Chen, Y.; He, X.; Ma, B.; Liu, K.; Gao, T.; Niu, W.*; Guo, J.* “Noncanonical amino acid mutagenesis in response to recoding signal-enhanced quadruplet codons” Nucleic Acids Res. 2022.

40. He, X.; Ma, B.; Chen, Y.; Guo, J*.; Niu, W.* “Genetic Encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells.” Chem. Commun. 2022, 58, 5897-5900.

39. Niu, W.; Guo, J.* “Genetic code expansion through quadruplet codon decoding” J. Mol. Biol. 2021.

38. Lawrie, J.; Waldrop S.; Morozov, A.; Niu, W.; Guo, J. “Engineering of a small protein scaffold to recognize sulfotyrosine with high specificity.” ACS Chem. Biol. 2021, 16, 1508-1517.

37. He, X.; Guo, J.*; Niu, W.* “Studying protein tyrosine O-sulfation in mammalian cells with genetically encoded sulfotyrosine” Curr. Protoc. 2021.

36. He, X.; Chen, Y.; Beltran, D.G.; Kelly, M.; Ma, B.; Lawrie, J.; Wang, F.; Dodds, E.; Zhang, L.; Guo, J.*; Niu, W.* "Functional genetical encoding of sulfotyrosine in mammalian cells" Nature Communications 202011:4820.

35. Kramer, L.; Le, X.; Rodriguez, M.; Wilson, M.A.; Guo, J.; Niu, W.* "Engineering carboxylic acid reductase (CAR) through a whole-cell growth-coupled NADPH recycling strategy" ACS Synthetic Biology 20209, 1632-1637.

34. Niu, W.*; Willett, H.; Mueller, J.; He, X.; Ma, B.; Guo, J. “Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT2440” Metabolic Engineering 2020, 59, 151-161.

33. Nogales, J.; Mueller, J.; Gudmundsson, S.; Canalejo, F.J.; Duque, E.; Monk, J.; Feist, A.M.; Ramos, J.L.; Niu, W.; Palsson, B.O. “High-quality genome-scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities” Environmental Microbiology 2020, 22, 255-269. ( equal contribution)

32. Kramer, L.; Le, X.; Hankore, E.D.; Wilson, M.A.; Guo, J.; Niu, W.* "Engineering and characterization of hybrid carboxylic acid reductases" Journal of Biotechnology 2019, 304, 52-56.

31. Niu, W.*; Kramer, L.; Mueller, J.; Liu, K.; Guo, J. “Metabolic engineering of Escherichia coli for the de novo stereospecific biosynthesis of 1,2-propanediol through lactic acid” Metabolic Engineering Communications 2019, 8, doi.org/10.1016/j.mec.2018.e00082.

30. Hankore, E.; Zhang, L.; Chen, Y.; Niu, W.; Guo, J.* “Genetic incorporation of noncanonical amino acids using two mutually orthogonal quadruplet codons”, ACS Synthetic Biology, 2019, 8, 1168-1174.

29. Liu, Y.; He, X.; Lim, W.; Mueller, J.; Lawrie, J.; Kramer, J.; Guo, J.; Niu, W.* “Deciphering molecular details in the assembly of alpha-type carboxysome” Scientific Reports 2018, doi: 10.1038/s41598-018-33074-x.

28. Kramer, L.; Hankore, E. D.; Liu, Y.; Liu, K.; Jimenez, E.; Guo, J.*; Niu, W.* “Characterization of Carboxylic Acid Reductases for biocatalytic synthesis of industrial chemicals” ChemBioChem 2018, 19, 1452-1460.

27. Chen, Y.; Wan, Y.; Wang, N.; Yuan, Z.; Niu, W.*; Li, Q.*; Guo, J.* “Controlling the replication of a genomically recoded HIV-1 with a functional quadruplet codon in mammalian cells” ACS Synthetic Biology 2018, 7, 1612-1617.

26. Shang, X.; Chen, Y.; Wang, N.; Niu, W.; Guo, J.* “Oxidation-induced generation of a mild electrophile for proximity-enhanced protein–protein crosslinking” Chemical Communications 2018, 54, 4172 – 4175.

25. Lawrie, J.; Song, X.; Niu, W.*; Guo, J.* “A high throughput approach for the generation of orthogonally interacting protein pairs” Scientific Reports 2018, 8, doi.org/10.1038/s41598-018-19281-6.

24. Shang, X., Lai, R.; Song, X.; Li, H.; Niu, W.; Guo, J.* “Improved photoinduced fluorogenic alkene-tetrazole reaction for protein labeling” Bioconjugation Chemistry 2017, 28, 2859-2864.

23. Shang, X.; Wang, N.; Cerny, R.; Niu, W.; Guo, J.* “Fluorescent protein-based turn-on probe through a general protection-deprotection design strategy” ACS Sensors 2017, 2, 961-965.

22. Yuan, Z.; Wang, N.; Kang, G.; Niu, W.*; Li, Q.*; Guo, J.* “Controlling multi-cycle replication of live-attenuated HIV-1 using an unnatural genetic switch” ACS Synthetic Biology 2017, 6, 721-731.

21. Shang, X.; Song, X.; Faller, C.; Lai, R.; Li, H.; Cerny, R.; Niu, W.; Guo, J.* “Fluorogenic protein labeling using a genetically encoded unstrained alkene” Chemical Science 2017, 8, 1141-1145.

20. Wang, N.; Yuan, Z.; Niu, W.*; Li, Q.*; Guo, J.* “Synthetic biology approach for the development of conditionally replicating HIV-1 vaccine” Journal of Chemical Technology and Biotechnology 2017, 92, 455-462.

19. Ju, T.; Niu, W.*; Guo, J.* “Evolution of SH2 domain to recognize sulfotyrosine” ACS Chemical Biology 2016, 11, 2551-2557.

18. Song, X., Shang, X.; Ju, T.; Niu, W.*, Guo, J.* “A photoactivable Src homology 2 (SH2) domain” RCS Advances 2016, 6. 51120-51124.

17. Wang, N.; Shang, X.; Cerny, R.; Niu, W.*; Guo, J.* “Systematic evolution and study of UAGN decoding tRNA in a genomically recoded bacteria” Scientific Reports 2016, 6, doi.org/10.1038/srep21898.

16. Cao, J.; Cheng T.; Zhao, G.; Niu, W.; Guo, J.; Xian, M.; Liu, H.* “Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose” BMC Biotechnology 2016, 16, doi: 10.1186/s12896-016-0257-x.

15. Niu, W.*; Guo, J. “Stereospecific microbial conversion of lactic acid into1,2-propanediol” ACS Synthetic Biology. 2015, 4, 378-382.

Prior to Department of Chemical and Biomolecular Engineering at UNL

14. Tong, W.; Xu, Y.; Xian, M.; Niu, W.; Guo, J.; Liu, H.; Zhao, G.* “Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coliApplied Microbiology Biotechnology 2016, 100, 4901-4907.

13. Cao, Y.; Niu, W.; Guo, J.; Xian, M.; Liu, H.* “Biotechnological production of 1,2,4-butanetriol: an efficient process to synthesize energetic material precursors from renewable biomass” Scientific Reports 2015, 5, doi.org/10.1038/srep18149.

12. Wang, N.; Ju, T.; Niu, W, Guo, J.* “Fine-tuning Interaction between Aminoacyl-tRNA Synthetase and tRNA for Efficient Synthesis of Protein Containing Unnatural Amino Acids” ACS Synthetic Biology 2015, 4, 207-212.

11. Wang, N.; Li, Y.; Niu, W.; Sun, M.; Cerny, R.; Li, Q.; Guo, J.* “Construction of a live-attenuated HIV-1 vaccine through genetic code expansion” Angewandte Chemie International Edition 2014, 53, 4867-4871.

10. Niu, W.; Guo, J.* “Expanding the chemistry of fluorescent protein biosensors by genetic incorporation of unnatural amino acids” Molecular BioSystems 2013, 9, 2961-2970.

9. Niu, W.; Schultz, P. G.; Guo, J.* “An expanded genetic code in mammalian cells with a functional quadruplet codon” ACS Chemical Biology 2013, 8, 1640-1645.

8. Ju, T.; Niu, W.; Ronald Cerny; Bollman, J.; Roy, A. Guo, J.* “Molecular recognition of sulfotyrosine and phosphotyrosine” Molecular Biosystems 2013, 9, 1829 - 1832.

7. Wang, G.; Xu, Z.; Chen, Z.; Niu, W.; Zhou, Y.; Guo, J.; Tan, L. “Sequential binding of large molecules to hairy MOFs” Chemical Communications 2013, 49, 6641-6643.

6. Wang, F.; Niu, W.; Guo, J.*; Schultz, P. G.* “Unnatural amino acid mutagenesis of fluorescent proteins” Angewandte Chemie International Edition 2012, 51, 10132-10135.   https://doi.org/10.1002/anie.201204668

5. Park, C. M.; Niu, W.; Liu, C.; Biggs, T. D.; Guo, J.; Xian, M. “A proline-based phosphine template for Staudinger ligation” Organic Letters 2012, 14, 4694-4697.

Prior to University of Nebraska-Lincoln

4. Yim, H.*; Haselbeck, R.*; Niu, W.*; Pujol-Baxley, C.*; Burgard, A.*; Boldt, J.; Khandurina, J.; Trawick, J. D.; Osterhout, R. E.; Stephen, R.; Estadilla, J.; Teisan, S.; Schreyer, H. B.; Andrae, S.; Yang, T. H.; Lee, S. Y.; Burk, M. J.; Van Dien, S. “Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol” Nature Chemical Biology 2011, 7, 445-452.  * These authors contributed equally to this work.  (This article was highlighted in this issue of Nature Chemical Biology)

3. Niu, W.; Molefe, M. N.; Frost, J. W.* “Microbial synthesis of the energetic material precursor 1,2,4-butanetriol” Journal of the American Chemical Society 2003, 125, 12998-12999.

2. Niu, W.; Draths, K. M.; Frost, J. W.* “Benzene-free synthesis of adipic acid” Biotechnology Progress 2002, 18, 201-211.

1. Niu, W.; Wu, Yi; Sui, Sen-Fang “Orientation of membrane-bound melittin studied by a combination of HPLC and liquid secondary ion mass spectrometry” IUBMB Life 2000, 50, 215-219.