The GMP fermentation suite features a 200 L fermentor (150 L working volume) and associated equipment for high cell density bacterial and yeast production of secreted or intracellular proteins. Fixed-angle rotor centrifugation, disc-stack centrifugation or tangential flow filtration (TFF) are available for culture harvest. To lyse cells, a homogenizer and a microfluidizer are available.
The purification suite features all the necessary downstream processing equipment to purify either intracellular or secreted proteins. Downstream process equipment includes a chromatography skid (flow rates ranging from 0.6 – 12 Lpm), as well as multiple TFF systems for concentration/diafiltration operations. Chromatography columns from 10 cm to 45 cm accommodate a wide range of process volumes.
GMP Equipment
Equipment in the GMP Facility consists of equivalent or scaled-up versions of equipment used in the Fermentation and Purification Development Laboratories.
- Bioengineering P200 Fermentation System
- AFC 903 5L Seed Fermentor
- YSI 2700 Bioanalyzer
- Westfalia CSC-20 Disc-Stack Centrifuge
- Niro Arite NS3006 VHP Homogenizer
- Microfluidics M110EH Microfluidizer
- Sani-matic CIP skid
- NC-SRT Model 50 Membrane System (2)
- NC-SRT Chromatography Skid
- NC-SRT Model 10 Membrane System
- NC-SRT Model 5 Membrane System
- 200 L Buffer Prep Station
- 500 L Buffer Prep Station
- Various Stainless Steel processing tanks ranging from 100 L to 660 L
- Various Chromatography Columns ranging from 10 cm to 45 cm Diameter