Angela K. Pannier, Ph.D.

Biomedical Engineer and Professor William E. Brooks Engineering Leadership Fellow
Academic Degrees
  • Ph.D., Biological Sciences, Northwestern University
  • M.S., Biological Systems Engineering, University of Nebraska-Lincoln
  • B.S., Biological Systems Engineering, University of Nebraska-Lincoln
  • 60% Reseach
  • 40% Teaching
Areas of Research and Professional Interest
  • Nonviral Gene Delivery
  • Protein-Cell-Biomaterial Interactions
  • Tissue Engineering
  • Stem Cell Differentiation
Teaching Interests
Honors and Awards
  • College of Engineering William E. Brooks Leadership Fellow (2014)
  • IANR Dinsdale Family Faculty Award (2012)
  • Gamma Sigma Delta (2011)
  • Honorary Member of Black Masque Chapter of Mortar Board (Faculty Inductee, 2011)
  • American Heart Association Scientist Development Grant (2010)
  • Nebraska EPSCoR First Award (2008)
  • Acta Biomaterialia Author Award (2005)

Selected Publications

Refereed Journal Articles

  • Martin TM, Wysocki BJ, Wysocki TA, Pannier AK.  2015. Identifying intracellular pDNA losses from a model of nonviral gene delivery. IEEE Transactions on NanoBioscience. 14(4): 455-464.
  • Kasputis T, Pieper A, Rodenhausen KB, Schmidt D, Sekora D, Rice C, Franke-Schubert E, Schubert M, Pannier AK. 2015. Use of precisely-sculptured thin film (STF) substrates with general ellipsometry to determine spatial distribution of adsorbed fibronectin to nanostructured columnar topographies and effect on cell adhesion. Acta Biomaterialia. 18:88-99.
  • Martin TM, Plautz SA, Pannier AK. 2015. Temporal endogenous gene expression profiles in response to lipid-mediated transfection. Journal of Gene Medicine. 17(1-2): 14-32.
  • Martin TM, Plautz SA, Pannier AK. 2015. Temporal endogenous gene expression profiles in response to polymer-mediated transfection and profile comparison to lipid-mediated transfection. Journal of Gene Medicine. 17(1-2):  33-53.
  • Martin TM and Pannier AK. 2015. Molecular Mechanisms of Nonviral Gene Delivery. In: Wang P and Liu Y (eds) The World Scientific Encyclopedia of Nanomedicine and Bioengineering: Nanotechnology in Gene Delivery.
  • Kasputis T, Farris E, Guerreiro GV, Pannier AK. 2015. Substrate-Mediated Gene Delivery. In: Wang P and Liu Y (eds) The World Scientific Encyclopedia of Nanomedicine and Bioengineering: Nanotechnology in Gene Delivery.
  • Wysocki BJ, Wysocki TA, Martin TM and Pannier AK. 2014. Simulation supported estimation of end-to-end transmission parameters in non-viral gene delivery. IEEE International Conference on Communications. 4179 – 4183.
  • Koenig M, Kasputis T, Schmidt D, Rodenhausen KB, Eichhorn KJ, Pannier AK, Schubert M, Stamm M, Uhlmann P. 2014. Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures. Analytical and Bioanalytical Chemistry. 406:7233-42.
  • Sargus-Patino CN, Wright EC, Plautz SA, Miles JR, Vallet JL, and Pannier AK. 2014. In vitrodevelopment of preimplantation porcine embryos using alginate hydrogels as a three-dimensional extracellular matrix. Reprod Fertil Dev. 26(7):943-53.
  • Kasputis T, Pieper A, Schubert M and Pannier AK. 2014. Dynamic Analysis of DNA Nanoparticle Immobilization to Model Biomaterial Substrates using Combinatorial Spectroscopic Ellipsometry and Quartz Crystal Microbalance with Dissipation. Thin Solid Films: ICSE-VI Proceedings. 571(3): 637-643.
  • Rodenhausen KB, Schmidt D, Kasputis T, Pannier AK, Schubert E, and Schubert M. 2012. Generalized ellipsometry in-situ quantification of organic adsorbate attachment within slanted columnar thin films. Optics Express. 20(5):5419-28.
  • Othman SF, Curtis ET, Plautz SA, Pannier AK, Butler SD, Xu H. 2012. Magnetic Resonance Elastography monitoring of tissue engineered constructs. NMR in Biomedicine. 25(3):452-63.
  • Sharp AT, Pannier AK, Wysocki BJ, Wysocki TA. 2012. A novel telecommunications-based approach to HIV modeling and simulation. Nano Communication Networks. 3(2): 129-137.
  • Plautz SA, Boanca G, Riethoven J-JM, Pannier AK. 2011. Microarray Analysis of Gene Expression Profiles in Cells Transfected with Nonviral Vectors. Molecular Therapy, 19(12):2144-51.
  • Rodenhausen KB, Kasputis T, Pannier AK, Gerasimov JY, Lai RY, Solinsky M, Tiwald TE, Wang H, Sarkar A, Hofmann T, Ianno N, and Schubert M. 2011. Combined optical and acoustical method for determination of thickness and porosity of transparent organic layers below the ultra-thin lm limit. Review of Scientific Instruments. Oct; 82(10):103111.
  • Rodenhausen KB, Duensing BA, Kasputis T, Pannier AK, Hofmann T, Schubert M, Tiwald TE, Solinsky M, and Wagner M. 2011. In-situ monitoring alkanethiol self-assembled monolayer chemisorption with combined spectroscopic ellipsometry and quartz crystal microbalance techniques. Thin Solid Films. 519(9): 2817-2820.
  • Singh D1, Pannier AK, Zempleni J. 2011. Identification of holocarboxylase synthetase chromatin binding sites using the DamID technology. Anal Biochem. 413(1):55-9.
  • Bellis AD, Bernabé BP, Weiss MS, Yarrington ME, Barbolina MV, Pannier AK, Jeruss JS, Broadbelt LJ, Shea LD. 2011. Cellular arrays for large-scale analysis of transcription factor activity. Biotechnol Bioeng. 108(2):395-403.
  • Pannier AK, Wieland JA, Shea LD. 2008. Incorporation of polyethylene glycol into self-assembled monolayers enhances substrate-mediated gene delivery by nonspecifically-bound complexes. Acta Biomat. 4:26-39.
  • Pannier AK, Ariazi EA, Bellis AD, Bengali Z, Jordan VC, Shea LD. 2007. Bioluminescence imaging for assessment and normalization in transfected cell arrays. Biotechnol Bioeng. 98:486-97.
  • Pannier AK, Anderson BC, Shea LD. 2005. Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning. Acta Biomat. 1:511-522.
  • Bengali Z, Pannier AK, Segura T, Anderson BC, Jang JH, Mustoe TA, Shea LD. 2005. Gene delivery through cell culture substrate adsorbed DNA complexes. Biotechnol Bioeng. May 5; 90(3):290-302.
  • Pannier AK, Shea LD. 2004. Controlled release systems for DNA delivery. Mol. Ther. 10: 19-26.
  • Pannier AK, Arora V, Iversen PL, Brand RM. 2004. Transdermal delivery of phosphorodiamidate Morpholino oligomers across hairless mouse skin. Int. J. Pharm. 275:217-226.
  • Pannier AK, Brand RM, Jones DD. 2003. Fuzzy modeling of skin permeability coefficients. Pharm. Res. 20: 143-148.

Book Chapters

  • Pannier AK and Segura T. 2012. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles. In: Ogris M and Oupicky D (eds) Nanotechnology for Nucleic Acid Delivery (in press).


  • Segura T, Shea LD, Pannier AK, Bengali Z, Jang JH, Chung P, and Anderson B. Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No. 7,029,697. April 18, 2006.