Gustavo Larsen

Contact Information:

City Campus (Lincoln)
207O OTHM
(402) 472-9805
glarsen1@unl.edu

Professor

Academic Degrees

  • Ph.D., Yale University, 1992
  • B.S., University de Mar del Plata, 1985

Areas of Research and Professional Interest

  • Catalysis
  • Adsorption
  • Materials Design
  • Nanostructured materials for biomedical applications

Research Profiles:

Courses Taught

About Gustavo Larsen

Dr. Larsen has been a Professor of Chemical and Biomolecular Engineering at the University of Nebraska-Lincoln since 1993. He completed his B.S. in Chemistry from the University of Mar Del Plata (Argentina) in 1985 and his Ph.D. in Chemical and Biomolecular Engineering from Yale University in 1992.

Dr. Larsen has devoted his research career to two broad fields, namely catalysis and adsorption, and the scalable production and testing of nanomaterials for biomedical applications. He is a highly cited author in both areas of materials engineering, and is also an inventor and patent holder of related technologies.

An innovation that is finally catching up: Needleless centrifugal electrospinning (NCES)

Sixteen years ago, Dr. Larsen and his team patented a device that used high-throughput, needleless centrifugal electrospinning (NCES) to make biocompatible ultrafine fibers. With a commercial angle in mind, Dr. Larsen began making nonwoven fibrous mats for hemostasis applications with such a device [1], and at production rates normally two orders of magnitude larger than conventional single-nozzle electrospinning. Automated machines capable of producing rolls of nonwoven fibers were built and used for many years, with funding from the U.S. Army and the Department of Energy. A key innovation was not even a delivery orifice was necessary to produce electrified liquid jets, which just formed from on open liquid surfaces.

In essence, a fiber-forming liquid precursor is fed perpendicularly at the center of a flat metal disk (typically 1-2 in diameter) rotating at high speed (1,000-100,000 rpm). A cylindrical coaxial collector drum, typically about 40-70 cm in diameter is used to deposit the electrospun fibers, as electrified liquid jets are ejected off the edge of the rotating disk and on solvent evaporation, solidification into submicron fibers occur [1]. The disk is also made to move vertically to create a rather homogeneous fibrous layer. Intriguingly, such a device (or straightforward variations of it) have been described in a number of much more recent patents -that we do not need to cite here in the interest of brevity- and showcased as revolutionary innovations, lending credence to the old adage that science and engineering are often plagued with “wheel reinvention”.

As one example, the figures below show nanofibers made using the NCES device from a liquid precursor comprising 15% wt% (poly)lactic acid, MW = 170 kDa (CorbionTM), in ethyl acetate.
A - Feed flow rate: 300 ml/hr, Voltage: 15kV, Rotational speed: 35000rpm
B - Feed flow rate: Voltage: 400 ml/hr, Rotational speed: 20kV, 35000rpm

Image from Larsen research          Image from Larsen research

[1] Gustavo Larsen, Rubén Spretz and Raffet Velarde-Ortiz, “Method of Manufacturing Fibrous Hemostatic Bandages”, U.S. Patent 8,257,778 (Priority date: 03-28-2006).

Experience

Projects:

  • Electrohydrodynamics as a tool for large-scale production of nanomaterials for medical applications such as controlled release of drugs and surgical bandages.
  • Catalysis and adsorption: oxidation, isomerization and biocatalysis. Pore size control via use of organic templates.

Memberships and Positions:

  • Active in American Institute of Chemical Engineers (AIChE), Member 1993-present
  • Editorial Board Member Applied Catalysis A (Elsevier), 2003-2007
  • Consultant for ABB Lummus Global, Inc; Kraft food, Inc.
  • Proposal review panelist for multiple Federal agencies
  • Founder LNKChemsolutions, nanotech company with nanoparticle and nanofiber applications as core businesses

Honors and Awards

  • National Science Foundation MCAA Award, 2002
  • National Science Foundation CAREER Award, 1998
  • Nominated for the University-wide Teaching Award, 1995
  • Yale University Prize Teaching Fellow, 1991
  • Henry Prentiss Becton Prize for Excellence in Engineering Research, 1992

Selected Publications

  • G. Larsen, “Discussion: On Danckwerts’ boundary conditions for the plug-flow with dispersion/reaction model”, Chem. Eng. Comm., published online April 01, 2021, https://doi.org/10.1080/00986445.2020.1835876

  • D. J. Voce, G. M. Bernal, L. Wu, C. D. Crawley, W. Zhang, N. M. Mansour, K. E. Cahill, S. J. Szymura, A. Uppal, D. R. Raleigh, R. Spretz, L. Núñez, G. Larsen, N. N. Khodarev, R. R. Weichselbaum and B. Yamini, “Temozolomide Treatment Induces IncRNA MALAT1 in NF-kB and p53 Codependent Manner in Gliobastoma”, Cancer Res. 79(10), 2536-2548 (2019).

  • J. Ragusa, D. González, S. Li, S. Noriega, M. Skotak and G. Larsen, “Glucosamine/L-lactide Copolymers ad Potential Carriers for the Development of Sustained Rifampicin Release System Using Mycobacterium Smegmatis as a Tuberculosis Model”, Helyon 5(4): e01539, April 2019.

  • U. R. Yanala, J. M. Johanning, I. I. Pipinos, R. R. High, G. Larsen, W. H. Velander and M. A. Carlson, “Fluid Administration Rate for Uncontrolled Intraabdominal Hemorrhage in Swine”, PLoS One 13(11): e0207708, November 2018.

  • J. S. Young, G. Bernal, S. P. Polster, L. Núñez, G. Larsen, N. Mansour, M. Podell and B. Yamini, “Convection-enhanced delivery of polymeric nanoparticles encapsulating chemotherapy in canines with spontaneous supratentorial tumors”, World Neurosurg. 117, e698-e704, September 2018.

  • D. González, J. Ragusa, P. C. Angeletti and G. Larsen, “Preparation and characterization of heparin-loaded of poly-caprolactone fibrous mats to prevent infection with human papilloma viruses”, PLoS One 13(7): e0199925, July 2018.

  • S. Capolla, N. Mezzaroba, S. Zorset, C. Tripodo, R. Mendoza-Maldonado, M. Granzotto, F. Vita, R. Spretz, G. Larsen, S. Noriega, E. Mansilla, M. Dal Bo, V. Gattei, G. Pozzato, L. Núñez and P. Macor, “A New Approach for the Treatment of CLL Using Chlorambucil/hydroxychloroquine-loaded anti-CD20 Nanoparticles”, Nano Res. 9(2), p. 537-548 (2016)

  • N. M. Mansour, G. M. Bernal, C. D. Crawley, K. E. Cahill, D. J. Voce, W. Zhang, R.Spretz, R. R. Weichselbaum, L. Núñez, G.. Larsen and B. Yamini, "Decoy Receptor 1 is a p50/Bcl3-regulated gene that blocks the anti-glioma effect of temozolomide", Cancer Res. 75(10), 2039-2048 (2015)

  • G. M. Bernal, M. J. LaRiviere, N. Mansour, P. Pytel, K. E. Cahill, D. J. Voce, S. Kang, R. Srpetz, U. Welp, S. E. Noriega, L. Núñez, G. Larsen, R. R. Weichselbaum and B. Yamini, “Convection Enhanced Delivery and In Vivo Imaging of Polymeric Nanoparticles for the Treatment of Malignant Glioma”, Nanomedicine: Nanotech., Biol. &. Med. 10(1), 149-157 (2014).

  • Yanala U. R., Johanning J. M., Pipinos I. I., Larsen G., Velander W. H., Carlson, M. A., “Development of a Fatal Noncompressible Truncal Hemorrhage Model with combined Hepatic and Portal Venous Injury in Normothermic Normovolemic Swine”, PLoS One, 9(9), e108293, Sep. 2014.

  • N. Mezzaroba, S. Zorzet, E. Secco, S. Biffi, C. Tripodo, M. Calvaruso, R. Mendoza-Maldonado, S. Capolla, M. Granzotto, R. Spretz, G. Larsen, S. Noriega, M. Lucafo, E. Mansilla, C. Garrovo, G. H. Marin, G. Baj, V. Gattei, G. Pozzato, L. Núñez and P. Macor, “New Potential Therapeutic Approach for the Treatment of B-Cell Malignancies Using Chlorambucil/Hydroxychloroquine Loaded Anti-CD20 Nanoparticles”, PLoS One 8(9), e74216 (2013).

  • Noriega, S. E., Hasanova, G. I., Schneider, M. J., Larsen, G. and Subramanian, A., “Effect of Fiber Diameter on the Spreading, Proliferation and Differentiation of Chondrocytes on Electrospun Chitosan Matrices”, Cells, Tissues & Organs 195, 207-221 (2012)

  • Mansilla E., Díaz Aquino V., Zambón D., Martire K., Marín G., Roque G., Drago H., Sturla F., Ichim T., Nuñez L., Larsen G., Riordan N., Spretz R., van Leeuwen M., Tau J.M., Soratti C., Ibar R., Maceira A., “Could Metabolic Syndrome, Lipodystrophy, and Aging Be Mesenchymal Stem Cell Exhaustion Syndromes?”, Stem Cells Int. 2011, published online May 2011, Article ID 943216, (2011).

  • Marin, G. H., E. Mansilla, N. Mezzaroba, S. Zorzet, L. Nunez, G. Larsen, J. M. Tau, A. Maceira, R. Spretz, C. Mertz, S. Ingrao, C. Tripodo, F. Tedesco, and P. Macor. “Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant B cells and on a human/mouse model of Burkitt lymphoma”, Curr Clin Pharmacol 5, 246 (2010).

  • M. Skotak and G. Larsen, "Visible Light Absorbing Biocompatible Oligomers Based on L-lactide and Aminosugars: Preparation and Characterization", Polymer Int. 59, 1331–1338 (2010).

  • M. Skotak, S. Noriega, G. Larsen and A. Subramanian, “Electrospun Cross-linked Gelatin Fibers with Controlled Diameter: The Effect of Matrix Stiffness on Proliferative and Biosynthetic Activity of Chondrocytes Cultured in Vitro”, J. Biomed. Mat. Res. A. 95A, 828-836 (2010).

  • M. Skotak and G. Larsen, "Visible Light Absorbing Biocompatible Oligomers Based on L-lactide and Aminosugars: Preparation and Characterization", Polymer Int. 59, 1331-1338 (2010).