Rajib Saha

Assistant Professor

Academic Degrees

  • Ph.D., Chemical Engineering, The Pennsylvania State University
  • M.S., Chemical Engineering, The Pennsylvania State University
  • B.S., Chemical Engineering, Bangladesh University of Engineering and Technology

News

Interesting research shows impressive prospect for higher ethanol blend in conventional vehicles!
Research led by us showed that non-flex-fuel vehicles could burn E-30 ethanol blends and get performance comparable to blends that use less of the corn-based fuel, such as E-10 or E-15. Multiple news outlets have featured the story, including EthanolProducer magazine, SDPB radio, KLKN TV, and Journal Star

 

Curriculum Vitae (CV):

Areas of Research and Professional Interest

  • Reconstruction and analysis of genome-scale and community models
  • Systems-level analysis of ‘omics’ data
  • Genetic redesign of non model organisms for biotechnological purposes
  • Development of genetic toolkit and engineering metabolic pathways
  • Redesign photosynthetic apparatus and carbon fixing mechanism

Research Profiles:

Courses Taught

About Rajib Saha

Dr. Rajib Saha is an Assistant Professor in the Department of Chemical & Bimolecular Engineering at UNL. Prior to his appointment at UNL, he worked as a post-doctoral research associate in the Himadri Pakrasi Lab in Biology department at Washington University in St. Louis. He graduated with his PhD and MS in Chemical Engineering from the Costas Maranas Lab at The Pennsylvania State University in 2014 and 2011, respectively. He obtained his Bachelor in Chemical Engineering from Bangladesh University of Engineering and Technology with the top position in his graduating class.

Throughout his multidisciplinary graduate research career, he has developed metabolic network models for photosynthetic organisms and subsequently utilized those for studying their physiology and also for metabolic engineering applications. His recent postdoctoral research includes study of light/dark behavior and development of efficient and tunable gene expression control system of a model cyanobacterial strain. His research interests include reconstruction and analysis of genome-scale and community models, systems-level analysis of ‘omics’ data, development of genetic toolkit and engineering metabolic pathways, and redesign photosynthetic apparatus and carbon fixing mechanism.

His lab, the Systems and Synthetic Biology Laboratory (SSBio) conducts research aimed at discovey and redesign of non-model microbes, plants and microbial consortia. We focus on both computational and experimental techniques to overcome the challenges in utilizing these organisms as platforms for producing bio-renewables or answering biological questions regarding metabolism, health and environment.

Experience

2016 -             Assistant Professor

                        Chemical & Biomolecular Engineering

                        University of Nebraska-Lincoln

2014 - 2016   Postdoctoral Research Fellow

                        Department of Biology, Washington University in St. Louis

                        Advisor: Dr. Himadri B. Pakrasi

2009-2014    Graduate Research Assistant

                        Department of Chemical Engineering, The Pennsylvania State University

                        Advisor: Dr. Costas Maranas

 2005-2009   Research Scholar

                        Department of Chemical and Biomolecular Engineering, National University of Singapore

Honors and Awards

Funding

  • NSF Molecular and Cellular Bioscience (MCB) Award
  • NSF Career Award
  • Nebraska Collaboration Initiative grants
  • Nebraska Corn Board award
  • Nebraska Ethanol Board award
  • Nebraska Center for Energy Sciences Research (NCESR) Energy Research grant
  • Center for Root & Rhizobiome Innovation (CRRI) grant

Selected Publications

  1. Islam, M. M., Schroeder, W., and Saha, R., "Kinetic Modeling of Metabolism: Present and Future", Current Opinion in Systems Biology, in Press, https://doi.org/10.1016/j.coisb.2021.04.003 (2021).
  2. Alsiyabi, A,. Solis. A.G., Cahoon. E. B., and Saha, R., "Dissecting the regulatory roles of ORM proteins in the sphingolipid pathway of plants, PLoS Comput Biol 17(1): e1008284 (2021).
  3. Long, D.S.,  Immethun, C.M., Vallecilla-Yepez, L., Wilkins, M.R., and Saha, R. "One step forward, two steps back: transcriptional advancements and fermentation phenomena in Actinobacillus succinogenes 130Z." bioRxiv preprint, doi: https://doi.org/10.1101/2020.12.31.424933, (2020).
  4. Zamani, E., Johnson, T. J., Chatterjee, S., Immethun, C., Sarella, A., Saha, R., and Dishari, S. K., "Cationic π-Conjugated Polyelectrolyte Shows Antimicrobial Activity by Causing Lipid Loss and Lowering Elastic Modulus of Bacteria", ACS Applied Materials and Interfaces, (2020).
  5. Islam, M. M., Sandhu, J., Walia, H., and Saha, R., "Transcriptomic data-driven discovery of global regulatory features of rice seeds developing under heat stress", Computational and Structural Biotechnology Journal, 18:2556-2567, (2020).
  6. Schroeder., W., and Saha, R., "Protocol for Genome-scale Reconstruction and Melanogenesis Analysis of Exophiala dermatitidis", STAR Protocol, (2020).
  7. Islam, M. M., Le, T., Daggumati, S. R., and Saha, R., "Investigation of microbial community interactions between lake Washington methanotrophs using genome-scale metabolic modeling", PeerJ, 8:e9464, (2020).
  8. Brown, B., Immethun, C., Wilkins, M., and Saha, R., "Rhodopseudomonas palustris CGA009 polyhydroxybutyrate production from a lignin aromatic and quantification via flow cytometry", Bioresource Technology Reports, 11:1000474 (2020).
  9. Schroeder., W., and Saha, R., "Introducing an Optimization- and explicit Runge-Kutta- based Approach to Perform Dynamic Flux Balance Analysis", Scientific Reports, 10: 9241 (2020).
  10. Schroeder., W., and Saha, R., "Computation-Driven Analysis of Model Polyextremo- tolerant Fungus Exophiala dermatitidis: Defensive Pigment Metabolic Costs and Human Applications", iScience, 23:100980, (2020).
  11. Islam, M. M., Thomas, V. C., Van Beek, M., Ahn, J., Alqarzaee, A. A., Zhou, C., Fey, P. D., Bayles, K. W., and Saha, R., "An integrated computational and experimental study to elucidate Staphylococcus aureus metabolism", npj Systems Biology and Application 6:3 (202o).
  12. Schroeder., W., and Saha, R., "OptFill: ATool for Infeasible Cycle-Free Gapfilling of Stoichiometric Metabolic Models", iScience 23:1 (2020).

Recent Presentations

  1. Brown, B. and Saha, R., “Conversion of Lignin into Biodegradable Plastic by Rhodopseudomonas palustris CGA009,” Proceedings of World Biomaterials Congress, Dec 11-15, 2020.
  2. Lamarre, Y., A. Aich, M. Islam, J. M. Scianni, A. C. S. Pinto, A. M. C. Tavassi, J. Elion, W. E. Nemer, R. Saha, S. Kashima, and D. T. Covas, “Leveraging plasma-derived exosomes for biomarker discovery in sickle cell disease: preparation for a large prospective study”, Hematology, Transfusion and Cell Therapy, 2020, 42:S2.
  3. Alsiyabi, A. and Saha, R., "Metabolic Modeling and Pulse Amplitude Modulation Fluorometry Elucidate the Interplay between Photosynthesis and Carbon Fixation in a Purple Non-Sulfur Bacterium", Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual., November 16-20, 2020.
  4. Immethun, C. and Saha, R., "Engineering a purple non-sulfur bacterium to expand symbiotic nitrogen fixation", Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual., November 16-20, 2020.
  5. Islam, M. M., Thomas, V. C., and Saha, R., “Optimization-Based Analysis of Comparative Metabolomic Data Identifies Regulatory Mechanisms in Staphylococcus Aureus mutants”, Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual., November 16-20, 2020.
  6. Brown, B.. and Saha, R., “Conversion of Lignin into Biodegradable Plastic by Rhodopseudomonas palustris CGA009,” Proceedings of World Biomaterials Congress, Dec 11-15, 2020.
  7. Brown, B.. and Saha, R., “Conversion of Lignin into Bioplastic by Rhodopseudomonas palustris,” Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual. Nov 15-20, 2020.
  8. Brown, B.. and Saha, R., “Rhodopseudomonas palustrisPolyhydroxyalkanoate Production from a Lignin Aromatic and Quantification via Flow Cytometry,” Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual. Nov 15-20, 2020.
  9. Schroeder, W. L. and Saha, R. “Computation-Driven Analysis of Model Polyextremotolerant Fungus Exophiala dermatitidis: Defensive Pigment Metabolic Costs and Human Applications”. Prerecorded Oral Presentation.2020 AICHE Annual Meeting, Virtual. Nov. 16, 2020.
  10. Schroeder, W. L. and Saha, R. “Introducing and Optimization- and Explicit Runge-Kutta- Based Approach to Perform Dynamic Flux Balance Analysis”. Prerecorded Oral Presentation. 2020 AICHE Annual Meeting, Virtual. Nov. 18, 2020.
  11. Schroeder, W. L. and Saha, R. “Predicting Enzyme Kinetics and Regulations from Dynamic Flux Balance Analysis Through Kinetic Optimization Using Integer Conditions”. Prerecorded Oral Presentation. 2020 AICHE Annual Meeting, Virtual. Nov. 19, 2020.
  12. Schroeder, W. L. and Saha, R.. “OptFill: A Tool for Infeasible Cycle-Free Gapfilling of Stoichiometric Metabolic Models”. Poster Presentation. 2020 AICHE Annual Meeting, Virtual. Nov. 20, 2020.
  13. Long, D., Immethun, C., Wilkins, M., and Saha, R., "Transcriptional Control of Gene Expression in Paraburkholderia sacchari", AIChE Annual Meeting 2020, Virtual, November 16-20, 2020.